IRDye 800CW Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG₁, IgG_2a, IgG_2b, and IgG₃, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:5,000 - 1:25,000
In-Cell Western Assay detection	1:800	1:200 - 1:1,200
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

P/N 925-32210: RRID AB_2687825
P/N 926-32210: RRID AB_621842

Example Data

IRDye<sup>®</sup> 800CW Goat anti-Mouse IgG Secondary Antibody data — **Western blot of cellular fractions from Staurosporine (STS) treated and non-treated Hela cells.** Lane 1: Odyssey^® One-Color Molecular Weight Marker (P/N 928-40000); Lane 2: human heart mitochondrial control (Mitosciences MS801-50); Lanes 4-6: control fractions (cytosolic, mitochondrial, and nuclear); Lanes 8-10: apoptotic (STS-treated) fractions (cytosolic, mitochondrial, and nuclear). Lysates were resolved on a 4-12% Bis-Tris gel and transferred to Odyssey Nitrocellulose (P/N 926-31090). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) and probed with ApoTrack mAb cocktail followed by detection with IRDye 800CW Goat anti-Mouse IgG (P/N 926-32210). Note: Cytochrome c is not present in the control cytosolic fraction and is present in the apoptotic cytosolic fraction.

IRDye® 800CW Goat anti-Mouse IgG Secondary Antibody