Revert 520 Total Protein Stain for Western Blot Normalization

Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment.

Revert 520 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 520 nm channel for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes.

The 200 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 500 mL size is sufficient for 50 mini Western blots.

Accurate, Reliable Western Blot Normalization with Revert 520 Stain

Revert 520 Total Protein Stain works with your existing Western blot workflow, without needing any additional reagents or special gels. Staining takes less than ten minutes.

Revert 520 staining of PVDF and nitrocellulose membranes — **Figure1. Rapid staining with Revert 520 Total Protein Stain.** Revert 520 Total Protein Stain provides highly efficient protein staining on nitrocellulose or Immobilon^® FL PVDF membranes in under 10 minutes. A dilution series using 20 µg to 313 ng of mouse heart tissue lysate was separated in 4-12% NuPAGE Bis-Tris gels, transferred to nitrocellulose or PVDF membranes, and then imaged using the 520 nm channel of an Odyssey M Imager.

Revert 520 absorbance and emission data chart — **Figure 2.** Absorbance and emission spectra of the working solution for Revert 520 Total Protein Stain.

Revert™ 520 Total Protein Stain for Western Blot Normalization

Accurate, Reliable Western Blot Normalization with Revert 520 Stain