Highly cross-adsorbed donkey anti-goat IgG (H+L) antibody conjugated to IRDye 800CW.
Immunogen
Goat IgG, whole molecule
Purity and Specificity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on electrophoresis, this antibody reacts with the heavy and light chains of goat IgG, and with the light chains common to most goat immunoglobulins. This antibody also reacts with sheep IgG. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with chicken. guinea pig, Syrian hamster, horse, human, mouse, rat, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
Applications
Highly recommended for:
- Western Blot
- In-Cell Western™ Assay
- On-Cell Western Assay
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
Formulation
IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Recommended Dilutions
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:5,000 - 1:25,000 |
| In-Cell Western Assay detection | 1:800 | 1:200 - 1:1,200 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-32214: RRID AB_2687553
- P/N 926-32214: RRID AB_621846
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