VRDye 490 Goat anti-Rabbit Secondary for Flow Cytometry

Immunogen

Rabbit IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled rabbit IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for immunofluorescence microscopy and flow cytometry applications.

Applications

Microscopy
Immunohistochemistry
Flow Cytometry

Formulation

VRDye 490 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Immunofluorescence Microscopy	1:400	1:100 - 1:1,000
Flow Cytometry	1:1,000	1:200 - 1:2,000
Other	User-optimized

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

Example Data

VRDye Goat anti-Rabbit customer image — **Immunofluorescence staining of tubulin protein in HeLa cells.** Cells were cultured on cover slips. After fixation and permeabilization, cells were incubated with rabbit anti-tubulin mAb (CST), followed by VRDye 490 Goat anti-Rabbit IgG (P/N 926-49020). Nuclei were stained with DAPI. Image acquired with Olympus IX81 microscope. Data provided by a LI-COR Customer.