IRDye 680RD Carboxylate is Non-Reactive and an Ideal Control.

Assays that use IRDye 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.

Carboxylate (non-reactive) form of IRDye 680RD is an ideal control.

Note: The carboxylate dye has no reactive group and cannot be used for labeling.

How is IRDye 680RD Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

Validation of optical agents for in vivo administration
Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

Timing of dye clearance from the animal's body
Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted ("free") dye after IRDye 680RD conjugation and purification.

Residual unreacted dye may cause:

Artificially high values when dye/protein (D/P) ratio is calculated
Increased background fluorescence in biological assays

Dye Structure

Properties

Chemical Formula: C₃₉H₄₁CIN₃Na₃O₁₁S₃
Molecular Weight: 928.37 g/mol
Exact Mass: 927.13

IRDye® 680RD Carboxylate