IRDye 800CW Goat anti-Human IgG Secondary Antibody

Highly cross-adsorbed goat anti-human IgG (H+L) antibody conjugated to IRDye 800CW.

Immunogen

Human IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using human IgG covalently linked to agarose. Based on ELISA, this antibody reacts with the heavy and light chains of human IgG. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, horse, and mouse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Highly recommended for:

Western Blot
In-Cell Western™ Assay
On-Cell Western Assay
Protein Array
Immunohistochemistry
Small Animal Imaging
Microscopy
2D Gel Detection
Tissue Section Imaging
Virus Titration Assay

Formulation

IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:5,000 - 1:25,000
In-Cell Western Assay detection	1:800	1:200 - 1:1,200
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

RRID

P/N 925-32232: RRID AB_2814904
P/N 926-32232: RRID AB_10806644

Example Data

IRDye® 800CW Goat anti-Human IgG Secondary Antibody data — **Detection of human IgG with IRDye 800CW Goat anti-Human IgG.** Serial dilutions of human IgG were spiked into C32 lysate (Santa Cruz P/N sc-2205). Samples were resolved on a 10% Bis-Tris gel and transferred to Odyssey^® Nitrocellulose membrane (P/N 926-31092). Membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with IRDye 800CW Goat anti-Human IgG (P/N 926-32232).

IRDye® 800CW Goat anti-Human IgG Secondary Antibody