Histone H3 Rabbit Monoclonal Antibody for Normalization

The Histone H3 primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting target proteins in nuclear extracts.

The expression of Histone H3, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, Histone H3 primary antibodies can be used for the detection of Histone H3 when performing multiplex Western blot detection.

Detect Histone H3 Rabbit Monoclonal Antibody with IRDye^® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Other options for housekeeping protein normalization

Reactivity and Specificity

Histone H3 antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.

Do not aliquot the antibody.

Properties	Histone H3 Rabbit Monoclonal Antibody (P/N 926-42219)
Species Cross-Reactivity	Human, mouse, rat, monkey
Target Molecular Weight	17 kDa
Isotype	Rabbit IgG
Specificity/Sensitivity	Detects endogenous levels of total Histone H3 protein (including isoforms H3.1, H3.2, and H3.3) and Histone H3 variant CENP-A. Does not cross-react with other core histones. May cross-react with bovine, chicken, D. melanogaster, hamster, xenopus, and zebrafish.
Immunogen	A synthetic peptide that corresponds to the carboxy terminus of the human histone H3 protein
Tested Applications	Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F)

RRID

P/N 926-42219: RRID AB_2814902

Detection of Histone H3 Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and Cos7 Lysates

**Histone H3 Rabbit Monoclonal Antibody detected in HeLa, NIH/3T3, and COS-7 Lysates.** Lysates were diluted from 2.5 μg to 156 ng. Lysates were separated on 4-12% Bis-Tris gels, electrophoresed at 200V for 45 minutes in MES Running Buffer, and transferred to nitrocellulose membranes in Tris-Glycine buffer at 100V for 65 minutes. Blots were blocked with Intercept^® (PBS) Protein-Free Blocking Buffer (P/N 927-90001), probed with Histone H3 Rabbit Monoclonal Antibody, and detected on an Odyssey^® CLx Imager.