The β-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of β-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, β-tubulin primary antibodies can be used for the detection of β-tubulin when performing two-color detection.
Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey, bovine |
| Target Molecular Weight | 55 kDa |
| Isotype | Rabbit IgG |
| Specificity/Sensitivity | Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin. |
| Immunogen | A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F) |
RRID
- P/N 926-42211: RRID AB_1850029
β-Tubulin Detected in Various Cell Lines
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