The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.
Detect β-Actin Mouse Primary Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | β-Actin Mouse Monoclonal Antibody (P/N 926-42212) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey, hamster |
| Target Molecular Weight | 45 kDa |
| Isotype | Mouse IgG2b |
| Specificity/Sensitivity | Detects endogenous levels of β-actin protein |
| Immunogen | A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F) |
RRID
- P/N 926-42212: RRID AB_2756372
Actin Used for Normalization
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SDS