IRDye 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody.

Immunogen

Mouse IgG₁ paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG₁ covalently linked to agarose. Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG₁. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG_2a, IgG_2b, IgG₃, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

Applications

Recommended for:

Western Blot
Protein Array
Immunohistochemistry
Microscopy
2D Gel Detection
Tissue Section Imaging

Not Recommended for:

Small Animal Imaging
In-Cell Western Assay
On-Cell Western Assay

Formulation

IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application	Recommended	Suggested Range
Odyssey Western blot detection	1:20,000	1:10,000 - 1:50,000
Other	User optimized

Optimum dilutions will vary and should be determined empirically.

Note: When using PVDF membranes for Western blotting applications, SDS (final concentration of 0.01 - 0.02%) and Tween^® 20 (final concentration of 0.1 - 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

RRID

P/N 926-68050: RRID AB_2783642

Example Data

IRDye® 680LT Goat anti-Mouse IgG1 Specific Secondary Antibody data — **Western blot analysis of PTEN expression in mouse PTEN transfected 293T whole cell lysate (lane 2) and non-transfected 293T lysate (lane 3).** Both lysates were loaded with 2 µg total protein per lane. Odyssey^® One-Color Molecular Weight Marker (P/N 928-40000) was loaded in lane 1. The blot was probed with mouse ant-PTEN (IgG_2b) (Santa Cruz P/N sc-133197) and mouse anti-GAPDH (IgG₁) (Abcam P/N ab8245) followed by detection with IRDye 800CW Goat anti-Mouse IgG_2b (P/N 926-32352) and IRDye 680LT Goat anti-Mouse IgG₁ (926-68050)

IRDye® 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody