α-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of α-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, α-tubulin primary antibodies can be used for the detection of α-tubulin when performing two-color detection.
Detect α-Tubulin Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
α-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.Do not aliquot the antibody.
| Properties | α-Tubulin Mouse Monoclonal Antibody (P/N 926-42213) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 52 kDa |
| Isotype | Mouse IgG1 |
| Specificity/Sensitivity | Detects endogenous levels of total α-tubulin protein. α/β-tubulin heterodimers form the tubulin subunit common to all eukaryotic cells. |
| Immunogen | Full-length chicken α-tubulin (purified from brain extracts) |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F) |
RRID
- P/N 926-42213: RRID AB_2637092
Generate an Autoquote
SDS