Plate Analysis Overview

When an image with a Plate Analysis is the current image, the Plate Analysis tab is shown on the ribbon. To apply a Plate Analysis to any image, click the quick launch button at the top of the window and select Plate from the drop-down menu. Or, open the Analysis tab and select Plate from the drop-down menu in the Type group. The last used parameters are used to create the new analysis.

Image Studio 5.0 Plate Analysis tab

Plate Group

Click the Plate Template button to choose, save, or delete a template that determines the Plate, Well, and Background method settings. Note: Templates are useful for quickly selecting the settings that are commonly used, however, you do not need to use a template to set up a Plate Analysis.

The Plate Template button resets to Custom when any of the Plate, Well, or Background method settings are changed. You can save the current settings to a new template.

  1. Click the Plate Template button and choose Save Current Template... from the drop-down list.
  2. Image Studio plate analysis save current template

  3. Enter a name for the new template and click OK.

    Image Studio 5.0 Template Name for Plate Array

  4. Choose the type of plate from the Define Plate Type drop-down list. You can choose from 6, 12, 24, 48, 96, 384, or 1536 well configurations. If your plate does not fall into one of these types, select Grid Analysis on the Analysis tab to set up a custom grid that fits your plate configuration.
  5. Adjust the size of the plate by selecting the bounding box and dragging the green arrows on each side. Move the entire plate by dragging the double arrows that appear when hovering on the image.

Well Group

Use the Circle or Square buttons in the Well group to select the shape of the wells. Use the Larger or Smaller buttons to incrementally adjust the size of the wells for a better fit.

Plate Background Group

The following options are available in the Background group.

  • Image Studio Set Background Icon Background list - Choose a background subtraction method from the Background list.
    • None - Unless data are going to be exported for analysis using a third-party software, do not choose None.
    • User-Defined - A well or wells must be designated as background.
  • Image Studio Assign Shape icon Assign Wells - When the User-Defined background method is chosen, this button is used to assign a well to be used for background calculation.
  • Image Studio Remove Background icon Unassign Wells - Removes a User-Defined background well from the background calculation.

Show Group

Use the controls in the Show group to specify what to display on the image. You can display Wells, Labels, Quantification, and Grid Border (see Show Group for further details).