PBS Blocking Buffer and PVDF Kits
TBS Blocking Buffer and PVDF Kits
Odyssey® Nitrocellulose Membranes
4X Protein Sample Loading Buffer and PVDF Membrane Kit
A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane.
LI-COR has evaluated many different membranes for Western blotting; examples of membrane performance can be seen in the figure below. There is typically more variability in PVDF membrane performance than for nitrocellulose membranes.
LI-COR offers high-quality, prescreened Immobilon®-FL PVDF, without lot-to-lot variability, packaged in affordable kits with the Intercept™ Blocking Buffer (PBS or TBS), or with 4X Protein Sample Loading Buffer. LI-COR also offers nitrocellulose membranes in both sheets and rolls.
| Product | P/N | What’s Included |
|---|---|---|
| Odyssey Nitrocellulose Membranes | 926-31090 | 0.22 µm; ten 7 cm × 8.5 cm sheets |
| Odyssey Nitrocellulose Membranes | 926-31092 | 0.22 µm; 30 cm × 3 m roll |
| PBS Blocking Buffer (500 mL) and PVDF Kit | 926-32098 | 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (PBS) Blocking Buffer |
| TBS Blocking Buffer (500 mL) and PVDF Kit | 926-32099 | 0.45 µm; 26.5 cm × 3.75 m roll + 500 mL Intercept (TBS) Blocking Buffer |
| 4X Protein Sample Loading Buffer and PVDF Membrane Kit | 926-31097 | 0.45 µm; 26.5 cm × 3.75 m roll + 15 mL 4X Protein Sample Loading Buffer |
| PBS Blocking Buffer (10 x 500 mL) and PVDF Kit | 829-32080 | 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (PBS) Blocking Buffer |
| TBS Blocking Buffer (10 x 500 mL) and PVDF Kit | 926-32100 | 0.45 µm; 26.5 cm × 3.75 m roll + 10 × 500 mL Intercept (TBS) Blocking Buffer |
