Technical Tips for Chemiluminescent Western Blot Imaging.

5 Technical Tips for Chemiluminescent Western Blotting Success with the C-DiGit Scanner

Tuesday, 27 January, 2015


tips for using the c-digit blot scanner,chemiluminescent western blots

In this short video, Jessica talks about 5 tips to help ensure that imaging chemiluminescent Western blots on the C-DiGit® Blot Scanner is a success - the first time and always!

 


Here is a recap of the tips of those five technical tips:

  1. Install Image Studio™ Software on your computer before connecting the C-DiGit Blot Scanner.
  2. Incubate using room temperature substrate.
  3. Wrap your blot so it stays wet during the scan.
    1. Remember to place your blot protein side down.
    2. If sensitivity is an issue, use WesternSure® PREMIUM or SuperSignal® West Femto Chemiluminescent Substrate.
  4. Start with high sensitivity scan for your first scan and then work from there.
  5. Image on the C-DiGit Scanner first and then exposure your film.

Happy Blotting!

Powered by Froala Editor