Multiplex Western blotting is a powerful tool that allows you to get more out of your Western blots. Multiplex detection becomes possible when you utilize the MPX™ (Multiplex) Blotting System and LI-COR IRDye® near-infrared fluorescent dye-labeled secondary antibodies.
Multiplex Westerns can be imaged on any of the Odyssey® Imagers and provide results for a possible maximum of 48 targets on a single membrane — 24 per channel with two-color detection — and the option for quantitative analysis, saving you time and reagents!
The MPX Blotting System can be used if you need to optimize:
- Primary antibodies - to determine the primary antibody that has the right specificity and the right dilution for use
- Antibody incubation times
- Blocking conditions - which blocking buffer will give you the optimum results
- Secondary antibodies - what dilutions is best to use without getting a lot of non-specific binding?
- Or just about anything else you need to optimize!
Watch this 4 minute video on how easy it is to get the most out of multiplexing with the MPX Blotting System.
You can also download the handy MPX Blotter User Guide.
Powered by Froala Editor