1

System Description

What it is, what it does, and how it does it

An Open System

The Flow Schematic

Equation Summary

Summary of Symbols

The System Components

2

Assembling the LI-6400XT

Putting it all together

Preparations

Using a Tripod

6400-01 CO2 Injector Installation

External Quantum Sensor Installation

Attaching the LED Light Source

6400-40 Leaf Chamber Fluorometer

Powering the LI-6400

Installing System Software

3

Guided Tours

Learning how to make it work

Before You Start

Tour #1: OPEN Overview

Tour #2: New Measurement Mode Basics

Tour #3: Controlling Chamber Conditions

Tour #4: Matching

Tour #5: Logging Data

Tour #6: Configuration Introduction

4

Making Measurements

The fundamentals of good measurements

Preparation Check Lists

Some Simple Experiments

Making Survey Measurements

Light Response Curves

CO2 Response Curves

Matching the Analyzers

Stability Considerations

Leaks

Operational Hints

7

Environmental Control

How OPEN controls chamber conditions

Support: LI-6400/XT Portable Photosynthesis System

Humidity Control

CO2 Control

Temperature Control

Light Control

11

Connecting to a Computer

Retrieving your data, and remote control

Support Software

Connecting with Ethernet

Connecting with RS-232

Remote Control

Getting Data From Serial Devices

18

Calibration Issues

The difference between 'data' and good data

CO2 and H2O Analyzers

Flow Meter

Zeroing the Leaf Temperature Thermocouple

6400-01 CO2 Mixer

Internal PAR Sensor (General)

6400-02(B) LED Source

6400-18 RGB LED Light Source

6400-40 Leaf Chamber Fluorometer

GaAsP Light Sensors

19

Maintenance and Service

The care and feeding of your new pet

Chemical Tubes

6400-03 Batteries

System Console

Real Time Clock

Cables

The Chamber Handle

Leaf Temperature Thermocouple

Leaf Chambers

LED Source Maintenance

Match Valve Maintenance

IRGA Maintenance

Servicing the External CO2 Source Assembly

Shipping the LI-6400

Useful Part Numbers

20

Troubleshooting

When things go wrong

Power On Problems

Real Time Clock Problems

New Measurements Mode Warning Messages

Unreasonable Results

Pump/Flow Problems

IRGA Problems

Match Valve Problems

6400-01 CO2 Mixer Problems

Light Source / Sensor Problems

6400-18 RGB Source

Chamber Problems

Finding Leaks

Useful Information

27

Leaf Chamber Fluorometer

Using the 6400-40 Leaf Chamber Fluorometer

Getting Started

Installing the LCF

Operational Summary

Using the MultiPhase Flash Protocol

Using LI6400XTerm and the LCF

Basic Experiments

Fluorescence AutoPrograms

Calibration Issues

LCF Troubleshooting

LCF Reference

28

Soil CO2 Flux Chamber

Using the 6400-09 Soil Chamber

Considerations

Attaching the Soil Chamber

Software

Making Measurements

Data Files

Troubleshooting the Soil Chamber

Maintenance