Scan Multiwell Plates on the Odyssey® M Imager

Once connected, click Multiwell Plate on the Choose Assay page to begin imaging a plate-based assay.

Choose Plate Assay

Choose the type of plate assay you want to image.

  • In‑Cell Western - Use this workflow to acquire In‑Cell Western or On-Cell Western plate images.

    Both the In‑Cell Western and On-Cell Western are quantitative immunofluorescence assays performed in microwell plates. Data analysis is based on whole-well fluorescence quantification.

  • Absorbance Assay

  • Cell Analysis - Cell Analysis refers to a broad range of assays for studying the function and localization of proteins in living and fixed cells. Cell Analysis covers assays that are not more precisely described as one of the other Multiwell Plate Assay types.

Select plate locations

Select the location of your plate(s) on the Odyssey M Scan Surface.

Choose detection options

  1. If you have multiple Scan Areas, click Edit Channels to choose detection options independently for each plate.

  2. Select the plate's well format.

  3. Click Scan to begin image acquisition.

Detection label reference

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Scan

After clicking Scan on the Choose detection options page, the Scan page will open with the image acquisition process already started.

When the scan has completed, the Ready symbol will appear.

Stop Scan

If you need to stop an image acquisition before the acquisition process completes, click Stop Scan.

This will stop all pending Acquisitions and will remove any partially completed Acquisitions.

Export Acquisition

Ensure the Include all acquisitions option is selected to export every image for every channel from this acquisition process.

Export to Analyze

Click Export to Analyze to export images to a single file. Import the file into Empiria Studio® Software for analysis.

Export to Publish or Present

Click Export to Publish or Present to export image file(s). Images exported using this method are not intended for analysis.

Images can be exported as high resolution TIFF files (for journal publications) or as PNG files appropriate for digital presentation (such as a slide show).

When you are exporting a single image, the default export image size shown in the "Export" dialog is the largest dimension (i.e., width or height) of the image set to 10 cm (4 in), and the other dimension is set according to the aspect ratio of the image. If you change the width or height in the dialog, the other dimension will update automatically to maintain the correct aspect ratio.

See View Images in the Image Gallery for more information.

Workflow

  • Click Start New to return to the beginning of the workflow to acquire another multiwell plate image.
  • Click Rescan to acquire new image(s) with the same settings.
  • Click Home to disconnect from the imager and return to the Home page.