Average and Median Background for Plate and Grid Analysis

The Average and Median background methods use space surrounding a feature as background.

Bkgnd Calculation: The average or median pixel intensity of a border region surrounding each spot will be used as the background subtracted from that spot.

Signal: The signal for each spot will be calculated by taking the Total for that spot and subtracting the product of the Bkgnd and the Area.

When using the Average or Median background methods, do not allow the border region designated as background to overlap a feature being quantified or autofluorescence from a plate.