Detection Label Reference

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Membrane

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 816 - 840 nm 700, 520, 488, RGB Epi
700 IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680 685 nm 721 - 740 nm 800, 520, 488, RGB Epi
520 Revert 520 Total Protein Stain, VRDye 549, Cy3, Alexa Fluor 546 520 nm 570 - 610 nm 800, 700, 488, RGB Epi
488 VRDye 490, FITC, Alexa Fluor 488 488 nm 519 - 543 nm 800, 700, 520, RGB Epi
RGB Epi Colorimetric Protein MW Markers NA NA 800, 700, 520, 488, Chemi, or 630 Epi, 525 Epi
630 Epi Pierce Reversible Protein Stain, TMB (blotting) 631 - 659 nm Clear Window RGB Epi
525 Epi Ponceau S, NBT/BCIP 500 - 530 nm Clear Window RGB Epi
Chemi HRP and AP chemiluminescent substrates NA NA 700, 520, RGB Epi

Gel (Protein and Nucleic Acid Gel)

Channel Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 816 - 840 nm
700 IRDye 680LT, IRDye 680RD, SYTO 60, Coomassie Stain 685 nm 721 - 740 nm
520 GelRed, Ethidium Bromide, Midori Green, Pro-Q Diamond Phosphoprotein Gel Stain 520 nm 570 - 610 nm
488 SYBR Safe, SYBR Green, GelGreen, GreenView, Pro-Q Emerald 488 Glycoprotein Gel Stain 488 nm 519 - 543 nm
488A SYPRO Ruby, SYPRO Orange, SYBR Gold 488 nm 570 - 610 nm
RGB Trans Colorimetric Protein MW Markers NA NA
630 Trans Coomassie Stain 631 - 659 nm Clear Window
470 Trans Silver Stain 447 - 472 nm Clear Window

Multiwell Plate Assays

In-Cell Western

Channel

Labels

Excitation

Emission
800 IRDye® 800CW, DyLight 800 785 nm 816 - 840 nm
700 IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5 685 nm 721 - 740 nm
520 VRDye 549, Cy3, Alexa Fluor 546, CellTag 520 Stain 520 nm 570 - 610 nm
488 VRDye 490, FITC, Alexa Fluor 488 488 nm 519 - 543 nm
Chemi HRP and AP substrates, luminescent assays NA NA

Any combination of up to four of the channels in the table can be selected per plate per acquisition. Different channels can be selected for different plates.

Cell Analysis

Channel Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 816 - 840 nm
700 IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5, SYTO 60 685 nm 721 - 740 nm
520 VRDye 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP), Propidium Iodide 520 nm 570 - 610 nm
488 VRDye 490, Green Fluorescent Protein (GFP), SYTOX Green 488 nm 519 - 543 nm
Chemi HRP and AP substrates, luminescent assays NA NA

Any combination of up to four of the channels in the table can be selected per plate per acquisition. Different channels can be selected for different plates.

Absorbance Assay

Channel Labels Excitation Emission
630 Trans TMB - non stopped, Bradford Protein Assay, Pierce 660nm Protein Assay, Modified Lowry Protein Assay 631 - 659 nm Clear Window
525 Trans OPD, BCA Protein Assay 500 - 530 nm Clear Window
470 Trans OPD, TMB - stopped, BCA Protein Assay 447 - 472 nm Clear Window
RGB Trans True color Transmissive RGB Color

Microscope Slide

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 816 - 840 nm 700, 520, 488, RGB Trans
700 IRDye 680RD, IRDye 680LT, Alexa Fluor 680 685 nm 721 - 740 nm 800, 520, 488, RGB Trans
520 VRDye 549, Cy3, Alexa Fluor 546, Propidium Iodide 520 nm 570 - 610 nm 800, 700, 488, RGB Trans
488 VRDye 490, FITC, Alexa Fluor 488, NucSpot 470, SYTOX Green 488 nm 519 - 543 nm 800, 700, 520, RGB Trans
RGB Trans H&E, Alcian Blue NA NA 800, 700, 520, 488

Custom

Channel Example Detection Labels Excitation Emission
800 IRDye 800CW, DyLight 800 785 nm 816 - 840 nm
700 IRDye 680LT, IRDye 680RD, IRDye 700, Revert 700 Total Protein Stain, Alexa Fluor 680, CellTag 700 Stain, DRAQ5 685 nm 721 - 740 nm
700A Custom 685 nm 816 – 840 nm
520 Revert 520 Total Protein Stain, CellTag 520 Stain, VRDye™ 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP) 520 nm 570 - 610 nm
520A DRAQ5 520 nm 721 - 740 nm
520B Custom 520 nm 816 - 840 nm
488 VRDye 490, FITC, Green Fluorescent Protein (GFP) 488 nm 519 - 543 nm
488A SYPRO Ruby, SYPRO Orange 488 nm 570 - 610 nm
488B StarBright Blue 700 488 nm 721 - 740 nm
488C Custom 488 nm 816 - 840 nm
Chemi HRP and AP chemiluminescent substrates NA NA
RGB Epi Colorimetric Protein MW Markers, Ponceau S NA NA
630 Epi Pierce Reversible Protein Stain, TMB (blotting) 631 - 659 nm Clear Window
525 Epi Ponceau S, NBT/BCIP 500 - 530 nm Clear Window
470 Epi Custom 447 - 472 nm Clear Window
RGB Trans H&E Stain, Alcian Blue, Coomassie stain, Silver stain NA NA
630 Trans Coomassie stain, TMB ELISA (non- stopped) 631 - 659 nm Clear Window
525 Trans Custom 500 - 530 nm Clear Window
470 Trans Silver stain, TMB – stopped ELISA, OPD 447 - 472 nm Clear Window

Membrane

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm 700
700 IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680 685 nm 710 – 730 nm 800

Gel

Channel Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm
700 IRDye 680LT, IRDye 680RD, Coomassie blue stain, SYTO 60 Stain 685 nm 710 – 730 nm

In-Cell Western

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm 700
700 IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5 685 nm 710 – 730 nm 800

Cell Analysis

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm 700
700 IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ5 685 nm 710 – 730 nm 800

Microscope Slide

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm 700
700 IRDye 680RD, IRDye 680LT 685 nm 710 – 730 nm 800

Custom

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm 700
700 IRDye 680RD, IRDye 680LT, Alexa Fluor 680 685 nm 710 – 730 nm 800

Membrane

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 816 - 846 nm 600, 700, Chemi
700 IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680 685 nm 716 - 746 nm 600, 800, Chemi
Chemi HRP and AP chemiluminescent substrate NA NA 600, 700, 800
600 Custom 520 nm 573 - 637 nm 700, 800, Chemi

Gel

Channel Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 816 - 846 nm
700 Coomassie Stain, SYTO 60 Stain, IRDye 680RD, IRDye 680LT 685 nm 716 - 746 nm
600 Ethidium Bromide, SYBR Safe 520 nm 573 - 637 nm
Channel Labels Excitation Emission
800 IRDye 800CW Optical Probes (or similar) 785 nm 816 - 846 nm
700 IRDye 680RD Optical Probes (or similar) 685 nm 716 - 746 nm
BLI Bioluminescence Detection N/A 450 - 650 nm
White White Light Detection White Light N/A

Membrane

Channels* Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 813 – 832 nm
700 IRDye 680LT, IRDye 680RD, Revert 700 Total Protein Stain, Alexa Fluor 680 685 nm 710 – 730 nm
520 Revert™ 520 Total Protein Stain, VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546 520 nm 570 – 610 nm
488 VRDye™ 490 Secondary Antibodies, FITC, Alexa Fluor 488 488 nm 538 – 552 nm

*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Gel

Channel* Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 813 – 832 nm
700 IRDye 680LT, IRDye 680RD, Syto 60, Coomassie stain 685 nm 710 – 730 nm
520 GelRed, Ethidium Bromide, Pro-Q Diamond Phosphoprotein Gel Stain 520 nm 570 – 610 nm
488 SYBR Safe, SYBR Green, Pro-Q Emerald 488 Glycoprotein Gel Stain 488 nm 538 – 552 nm
488A SYPRO Ruby, SYPRO Orange, SYBR Gold 488 nm 570 - 610 nm

*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Microscope Slide

Channel* Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 813 – 832 nm
700 IRDye 680LT, IRDye 680RD, Alexa Fluor 680 685 nm 710 – 730 nm
520 VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546, Propidium Iodide 520 nm 570 – 610 nm
488 VRDye™ 490 Secondary Antibodies, Alexa Fluor 488, NucSpot 488, STYOX Green 488 nm 538 – 552 nm

*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).

Multiwell Plate

Channel* Labels Excitation Emission
800 IRDye® 800CW, DyLight 800 785 nm 813 – 832 nm
700 IRDye 680RD, CellTag™ 700 Stain, Sapphire700™ Stain, DRAQ 5 685 nm 710 – 730 nm
520 CellTag™ 520 Stain, VRDye™ 549 Secondary Antibodies, Cy3, Alexa Fluor 546 520 nm 570 – 610 nm
488 VRDye™ 490 Secondary Antibodies, FITC, Alexa Fluor 488 488 nm 538 – 552 nm

*All channels shown are available in this workflow for the 4-laser Odyssey F (9180). The 700 and 800 channels are available for the 2-laser Odyssey F (9150).