 Improving Sensitivity with Odyssey Blots vs. Chemiluminescence Blots
Improve your Odyssey blot performance with the tips provided in this presentation.
Troubleshooting tips include:
- Changing software settings such as the Background, Linear Manual/Linear Auto, and Brightness and Contrast.
- Altering the assay chemistry by changing dilution factors, using different blocking buffers, and more.
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LATEST WEBINAR: Quantitative, Multiplexed Western Blot Detection with Infrared Fluorescence
– Dr. Amy Geschwender May 1, 2008 (56 minutes)
This presentation discusses the differences between fluorescent and chemiluminescent Western detection,
and illustrates how this technology is being used around the world.
Topics include:
- How near-infrared fluorescence differs from other Western blotting methods
- Linear ranges of detection for fluorescence and chemiluminescence
- Multiplexed Westerns
- Transitioning from ECL to fluorescence
- How this technology is being used
- Quantification and normalization
- Phosphoprotein analysis
- Validation of RNAi knockdown
- High throughput Western blotting
- In-gel Western detection without transfer
- Reagents, instruments, and software for fluorescent Western blotting
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Odyssey Infrared Imaging System - Dr. Amy Geschwender -
Jun 6, 2008
Previously featured on BioCompare, Dr. Geschwender, Director of Molecular Biology @ LI-COR Biosciences, introduces the Odyssey Infrared Imaging System with particular focus on it's application in Western Blotting and it's advantages when compare with the best chemiluminescence has to offer. |
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Characterizing GPCR Function - Dr. Christopher Kearn -
Jun 6, 2008
Dr. Christopher Kearn, University of Washington, discusses the effects of CB1 and CB2 cannabinoid receptor stimulation or inhibition and how the signaling of CB1 receptors through these various effectors is regulated. |
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Antibody FAQs - Dr. Kristi Ambroz -
Jun 6, 2008
Watch
a 9 minute question and answer presentation by Dr. Kristi Ambroz, LI-COR
Senior Scientist.
Questions Include:
• What are the advantages
of infrared detection?
• What
kinds of dye labeled antibodies can I use on the Odyssey/Aerius?
• Where
do I get infrared labeled antibodies? |
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Optimizing Signal Detection for Odyssey Western Blots -
Jun 6, 2008
A short instruction on how to troubleshoot missing and weak Odyssey Western Blot bands. |
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Optimizing Signal Detection for Odyssey Western Blots -
Jun 6, 2008
A short instruction on how to troubleshoot missing and weak Odyssey Western Blot bands.
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Odyssey Infrared Imaging System - Dr. Amy Geschwender -
Jun 6, 2008
Previously featured on BioCompare, Dr. Geschwender, Director of Molecular Biology @ LI-COR Biosciences, introduces the Odyssey Infrared Imaging System with particular focus on it's application in Western Blotting and it's advantages when compare with the best chemiluminescence has to offer. |
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