MPX^™ (Multiplexer) Blotting System

• Screen multiple samples and multiple targets on the same blot. • Will accommodate most common Western blotting procedures. • 24 independent channels provide a maximum of 48 targets with the Odyssey Imaging System. • Conserve precious antibody and reagents with lower volumes.
Screen multiple samples and multiple targets all on the same blot. No cutting. No stripping. No cross-contamination. Adapt your existing Western blotting procedure for comprehensive antibody screening with the MPX Blotting System. Ideal for any multiple-target Western blot application, the MPX increases throughput, maximizes efficiency and reduces relative overall cost. Quantitative analysis is possible with utilization of the Odyssey® and Odyssey optimized reagents.	Representative MPX Western Blot with one sample and multiple targets
Training video Click to view a short demonstration showing the suggested use of the MPX Blotting System. The video shows proper layout of the membrane, addition of antibodies by both single channel and multichannel pipettes, and tips and tricks for getting optimum results.

 

Application Versatility

The MPX can be incorporated into a variety of assays. Examples include: primary and secondary antibody screening, monoclonal antibody screening, signal transduction pathway analysis, and time course studies. However, it is possible to adapt nearly all common Western procedures that generate a PVDF or Nitrocellulose blot of 7 x 8.5 cm to the MPX format.

The unique collection of multiple-sample single- and dual-marker gel combs provides versatility for ideal sample/target combination. The combs are custom designed to maximize the number of channels per sample at each configuration. Alternatively, pre-cast gels can be used to generate blots for processing.

Simple, Convenient Workflow

Channel ports are conveniently spaced, staggered and beveled creating a simple workflow for using both standard and multi-channel pipettes. The low volume channel ports accommodate a maximum volume of 160 μl, thus conserving precious and costly antibody. The nesting dovetail joint design makes it easy to handle multiple-blot processing by allowing the continuous linkage of units.

Screen Up To 48 Targets on One Blot

Processing is as simple as clamping the blot into the MPX creating up to 24 independent channels. The range of usable channels per sample is relative to comb-size---from 24 on a single sample to four on a four-sample blot. Using LI-COR’s selection of IRDye® infrared dyes on the Odyssey provides a maximum of 48-targets on a single membrane— two per channel—and the option for quantitative analysis. Other detection methods, such as standard chemiluminescence and colormetrc methods can provide a maximum 24 targets.

 

Compatibility

The MPX is compatible with all common Western blotting procedures that generate a nitrocellulose or PVDF blot of 7 cm x 8.5 cm. It can be utilized with all standard chemiluminescence, colormetric, and infrared protocols. The channel ports are spaced to accommodate most common multichannel pipettes.

MPX vs Standard Western Blot Cost Comparison Using Near-Infrared Detection

Reagents	IR Detection 2-targets (15-well gel)	Chemiluminescence (15-well gel)		24-well MPX IR Detection
		1st -target	2nd-target
Blocker	7.80	7.80	7.80	5.20
Primary Antibody ß-Tubulin (Upstate 05-661) $350 (200 µg/mL, 200µL) ERK 1 (Santa Cruz SC-94) $229 (200 µg/mL,200µL)	34.50 18.32	34.50 ---	--- 18.32	6.90 1.04
Secondary Antibody (Recommended Dilutions: 1:15,000 for IR, 1:25,000 for Chemi, 1:5,000 for MPX)	0.82	0.09	0.09	0.08
Chemiluminescent Substrate (0.1 ml/cm2)	0.00	12.20	12.20	0.00
Film (2 sheets/blot)	0.00	7.71	7.71	0.00
Protein Markers (Recommended Amount: 1µL for IR; 10 µL for Chemi)	0.22	2.26	2.26	0.22
Total ($)	61.66	64.56	48.38	13.44
$ per Data Point	2.06	4.30	3.23	0.28