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The Advantages of Infrared Imaging

Quantitative

"The ability to quantitatively detect two targets unambiguously in the same sample lane makes analysis more accurate and increases experimental throughput "

 

arrow Quantitative, Two-Color Western Blot Detection with Infrared Fluorescence Schutz-Geschwender, et. al., LI-COR Biosciences, May 2004.

 

For Membrane and Plate-based Applications

Through its innovative use of fluorescently labeled antibodies rather than enzyme labels, infrared provides a broad, linear dynamic range to accurately detect strong and weak bands on the same Western blot. In contrast, the dynamic enzymatic nature of chemiluminescence only allows you to capture a “snapshot” of the enzymatic reaction and is highly dependent on timing and exposure, limiting linear range and offering only qualitative or semi-quantitative results. The accuracy and linearity of infrared detection allows researchers to be confident about differences seen in protein levels, and blots can be archived and imaged again months later, if needed


better scatter and absorbance Low Autofluorescence wide dynamic range
more Reduced Scatter and Absorbencies more Enhanced Detection more Wider Dynamic Range
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