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Direct Detection

"...200-fold greater sensitivity than previous studies with visible fluorophores and greater sensitivity than chemiluminescence."

 

arrow Quantitative, Two-Color Western Blot Detection with Infrared Fluorescence A. Schutz-Geschwender, et.al., LI-COR Biosciences, May 2004.

 

 

High Signal-to-Noise

In the visible wavelength range used by most fluorescent imaging systems, membranes and plastics produce high background due to light scattering and autofluorescence. This high background limits the sensitivity making use of visible fluorescence nearly impossible to detect low-abundance proteins.

At the infrared wavelengths both autofluorescence and light scatter are dramatically reduced. The result is the cleanest background, highest signal-to-noise ratios, and enhanced detection sensitivity compared to chemiluminescence.

wide dynamic range quantitative western better scatter and absorbance
more Wider Dynamic Range more Quantitative more Reduced Scatter and Absorbance
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